KMID : 1134820220510030229
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Journal of the Korean Society of Food Science and Nutrition 2022 Volume.51 No. 3 p.229 ~ p.236
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Immunostimulatory Activity of Allomyrina dichotoma Larva Extract through the Activation of MAPK and the NF-¥êB Signaling Pathway in Macrophage Cells
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Hong Jun-Pyo
Yoo Bo-Gyeong Lee Joung-Hee Song Ha-Yeon Byun Eui-Hong
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Abstract
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Macrophages play an important role in the innate immune system. Allomyrina dichotoma, an edible insect, has been in focus as an alternative protein source. However, the capacity of Allomyrina dichotoma larva hot water extracts (ADLE) as immune potentiators is still unknown. In this study, we investigated whether ADLE has immunostimulatory activity on macrophages. The ADLE treatment did not induce cytotoxicity in RAW 264.7 cells at doses ranging from 1 to 200 ¥ìg/mL. The ADLE treatment resulted in the secretion of pro-inflammatory cytokines (tumor necrosis factor-¥á and interleukin-6) in a dose-dependent manner and the production of nitric oxide in RAW 264.7 cells at doses of 100 and 200 ¥ìg/mL. Furthermore, ADLE (100 and 200 ¥ìg/mL) increased the expression of the surface molecules (CD80, CD86, major histocompatibility complex-¥°, and ¥±) in RAW 264.7 cells. ADLE-treated RAW 264.7 cells showed phosphorylation of mitogen-activated protein kinases (extracellular signal-regulated kinases, c-Jun amino-terminal kinases, and p38) and nuclear factor-kappa B (NF-¥êB) translocation. Collectively, these results indicate that ADLE exhibits immune-enhancing effects in macrophages, and thus has value as an immunomodulatory supplement.
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KEYWORD
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Allomyrina dichotoma larvae, immunostimulatory activity, macrophage, cytokine production, NF-¥êB
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